Lead Laboratory Technician And Manager Resume Example (University Of Utah) - Salt Lake City, Utah

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University of Utah - Lead Laboratory Technician and Manager Resume Example

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XXXX, Salt Lake City, Utah 84118 | H: XXX-XXXX-XXX | XXX-XXXX-XXX XXXX@XXXX.XXX
Professional Profile

Angela Freeman summary Intrinsically analytical Research Laboratory Technical, Supervisory and Managerial Professional in pursuit of employment within challenging and dynamic environment where skills and experience may be utilized toward laboratory success and growth, resulting in positive contribution toward society.

Highly Accomplished Research Scientist
  • Nine years practice perfecting extremely wide range of proficiencies in; genetic, molecular biological, biochemical, proteomic, cell biological and animal surgical technical methodologies (Laboratory Skills section)
  • Four publications plus two in progress
  • Clever utilization of PhD level literature research and prior knowledge for method selection
  • Deep understanding of molecular genetics and pathophysiological mechanisms
  • Specialist at verification of genetics and proteomics
  • Brilliant hypothesis formation and translation into research projects
  • Outstanding performance, individually or as part of a team
  • Communication Seven years fruitful Laboratory Supervision and Management Impeccable leadership of four research projects resulting in publication Successful contribution of innovative ideas to research projects Direction of entire laboratory of up to seven people, independently for 2/3 of its most productive year Culmination of beautiful technical writing, figure creation and design for publication and or presentation Maintenance of positive collaborative relationships with professional people Flawless and detailed SOP creation for students and employees of all levels
Education
BACHELOR OF SCIENCE, BIOLOGY WESTMINSTER COLLEGE OF SALT LAKE CITY Salt Lake City, Utah
Professional Experience
Lead Laboratory Technician and Manager Mar 2012 to 30/2013
University of Utah
  • Laboratory skills Molecular, Genetic, Proteomic, Cellular and Biochemical Techniques: PCR, quantitative real-time-PCR, reverse transcription, primer design and Southern hybridization DNA microarrays, in-situ hybridization and fluorescent probe synthesis Cloning, cDNA synthesis, plasmid construction DNA and RNA; extraction and purification Recombinant and native protein purification and analysis Transfection assays, translation table generation TUNEL labeling, FACS and flow cytometry DNA/RNA labeling with fluorescent or radioactive probes Northern and Southern hybridization Site directed mutagenesis and point mutation study HPLC, GC, UV-vis spectroscopy, mass spectrometry and capillary gel electrophoresis High throughput (WTSS), RFLP- SNP and Next Generation sequencing Sub-cellular fractionation Western hybridization, ELISAs and luciferase assays Immunohistochemistry; staining and fluorescence staining Histological tissue procurement, processing and sectioning Tissue culture; F258V, NIH3T3,.
Mar 2002 to Jan 2005
  • Directed, supervised and managed laboratory.
  • Purified and characterized the virulence factors of selected species of Mycoplasma and Ureaplasma employing microbiological, molecular, proteomic and animal surgical techniques.
  • For example; 2D SDS-page, then Edman degradation followed by HPLC and UV-vis for some purification and characterization steps.
  • Also, qRT-PCR, ELISAs and protein sequence analysis.
  • Analyzed and verified data, Completed technical writing and figure creation for publication, and or presentation.
  • Publications 2, 3.
  • Laboratory Technician and manager, Huntsman cancer institute at the university of utah, department of oncology.
Oct 1997 to Feb 2002
  • Directed, supervised and managed laboratory.
  • Studied leukemia and apoptosis.
  • Employed molecular, genetic, biochemical, proteomic, cellular and animal surgical techniques.
  • For example; genetic variant analysis, which led to the investigation of BCL-2 and BAX molecular mechanisms such as; site directed mutagenesis, subcellular fractionation and electron microscopy.
  • Combined chemotherapy with experimental compounds, and did HPLC and mass spectrometry for chemical analysis.
  • Analyzed and verified data.
  • Completed technical writing and figure creation for publication, and or presentation.
  • Publications 5, 6.